郭瑞庭,二级教授、博士研究生导师
电子邮件:guoreyting(at)hubu.edu.cn
研究领域:酶分子结构、机理研究、应用与改造
郭瑞庭教授通过结构生物学方法解析重要工业酶结构,阐释催化机理,进行酶分子理性设计,以改造酶的活性与特性。工作内容主要涵盖五个体系:(1) PET塑料降解酶; (2) 新型萜类合成酶;(3) 霉菌毒素分解酶; (4) 生物质降解酶;(5) P450 酶。目前已在Nature, Nature Reviews Chemistry, Nature Catalysis, Immunity, Nature Communications, Angew. Chem. Intl. Ed., JACS, ACS Catalysis, PNAS 等SCI期刊发表论文150余篇,超过30篇获选为封面文章,申请国内外专利近50个,已授权26个。2019年获天津市自然科学二等奖(第一完成人)。
二、教育背景
2003–2007 台湾大学生化科学所暨台湾中研院生物化学所,博士
2014–2017 台湾政治大学EMBA,获商学硕士
三、工作经历
2018年至今 湖北大学生命科学院、省部共建生物催化与酶工程国家重点实验室
2010–2018 中科院天津工业生物技术研究所,研究员、博导
2007–2017 年台湾基酵生物科技公司,研发总监/研发总顾问
2007–2009 美国The Scripps Research Institute,博士后研究
四、 承担科研项目情况
1. 湖北省洪山实验室重大项目、2022/11-2025/10、80万、在研、主持
2. 国家重大专项、2019/12-2024/11,552万、在研、主持
3. 湖北省创新群体、2020/3-2023/3,50万、在研、主持
4. 自然科学基金面上项目 (三次)、2015/01-2018/12、2019/01-2022/12、2023/01-2026/12,共198万、两项结题一项在研、主持
5. 863计划、2012/8-2015/12、项目9450万、已结题、项目首席科学家
6. 973计划、2011/01-2015/08、75万、已结题、主持
7. 中科院创新交叉团队、2017/1-2019/12、100万、已结题、团队负责人
8. 中科院重点部署项目、 2013/01-2015/12、235万、已结题、主持
9. 中科院***计划、2010年-2013年、260万、已结题、主持
10. 天津市科技项目、2011年-2014年、150万、已结题、主持
11. 企业合作开发项目 (东莞泛亚太)、2012年-2015年、255万、已结题、主持
五、 代表性论文
1. Yang, Y., # Cheng, S., # Zheng, Y., # Xue, T., Huang, J.-W., Zhang, L., Yang, Y., Guo, R.-T.,* Chen, C.-C.* Remodeling the polymer-binding cavity to improve the efficacy of PBAT-degrading enzyme. Journal of Hazardous Materials, 2023, Accepted. https://doi.org/10.1016/j.jhazmat.2023.132965
2. Li, H., # Huang, J.-W., # Dai, L., # Zheng, H., Dai, S., Zhang, Q., Yao, L., Yang, Y., Yang, Y., Min, J., Guo, R.-T.,* Chen, C.-C.* The structural and functional investigation into an unusual nitrile synthase. Nature Communications, 2023, Accepted.
3. Yuan, L., # Ma, X., # Yang, Y., # Qu, Y., Li, X., Zhu, X., Ma, W., Duan, J., Xue, J., Yang, H., Huang, J.-W., Yi, S., Zhang, M., Cai, N., Zhang, L., Ding, Q., Lai, K., Liu, C., Zhang, L., Liu, X., Yao, Y., Zhou, S., Li, X., Shen, P., Chang, Q., Malwal, S. R., He, Y., Li, W., Chen, C., Chen, C.-C., Oldfield, E., Guo, R.-T.,* Zhang, Y.* Phosphoantigens glue butyrophilin 3A1 and 2A1 to activate Vγ9Vδ2 T cells. Nature, 2023, 621(7980): 840-848.
4. Dai, L., # Niu, D., # Huang, J.-W., # Li, X., Shen, P., Li, H., Xie, Z., Min, J., Hu, Y., Yang, Y., Guo, R.-T.,* Chen, C.-C.,* Cryo-EM structure and rational engineering of a superefficient ochratoxin A-detoxifying amidohydrolase. Journal of Hazardous Materials, 2023, 458: 131836.
5. Li, Z., # Zhang, L., # Xu, K., # Jiang, Y., Du, J., Zhang, X., Meng, L.-H., Wu, Q., Du, L., Li, X., Hu, Y., Xie, Z., Jiang, X., Tang, Y.-J., Wu, R.,* Guo, R.-T.,*Li, S.* Molecular insights into the catalytic promiscuity of a bacterial diterpene synthase. Nature Communications, 2023, 14(1): 4001.
6. Li, X., # Shi, B., # Huang, J.-W., Zeng, Z., Yang, Y., Zhang, L., Min, J., Chen, C.-C.,* Guo, R.-T.* Functional tailoring of a PET hydrolytic enzyme expressed in Pichia pastoris. Bioresources and Bioprocessing, 2023, 10(1): 26.
7. Xin, L., # Min, J., # Hu, H., # Li, Y., # Du, C., Xie, B., Cheng, Y., Deng, X., Deng, X., Shen, K., Huang, J., Chen, C.-C., Guo, R.-T.,* Dong, C.,* Zhou, H.* Structure-guided identification of novel dual-targeting estrogen receptor α degraders with aromatase inhibitory activity for the treatment of endocrine-resistant breast cancer. European Journal of Medicinal Chemistry, 2023, 253: 115328.
8. Yang, Y., # Min, J., # Xue, T., # Jiang, P., Liu, X., Peng, R., Huang, J.-W., Qu, Y., Li, Xian., Ma, Ning., Tsai, F.-C., Dai, L., Zhang, Q., Liu, Y.,* Chen, C.-C.,* Guo, R.-T.* Complete bio-degradation of poly(butylene adipate-co-terephthalate) via engineered cutinases. Nature Communications, 2023, 14(1): 1645.
9. Li, A., # Wang, Q., # Song, X., # Zhang, X., Huang, J.-W., Chen, C.-C., Guo, R.-T., * Wang, B.,* Reetz, M. T.* Engineering of a P450-based Kemp eliminase with a new mechanism. Chinese Journal of Catalysis. 2023, 47: 191-199.
10. Zhang, X., # Shen, P., # Zhao, J., # Chen, Y., Li, X., Huang, J.-W., Zhang, L., Li, Q., Gao, C., Xing, Q., Chen, C.-C., Guo, R.-T.,* Li, A.* Rationally controlling selective steroid hydroxylation via scaffold sampling of a P450 family. ACS Catalysis, 2023, 13(2): 1280-1289.
11. Chen, Q., # Li, B., # Zhang, L., # Chen, X., Zhu, X., Chen, F., Shi, M., Chen, C.-C., Yang, Y., Guo, R.-T.,* Liu, W., Xu, J., Zheng, G.* Engineered imine reductase for larotrectinib intermediate manufacture. ACS Catalysis, 2022, 12(23): 14795-14803.
12. Dai, L., # Li, H., # Huang, J.-W., # Hu, Y., # He, M., Yang, Y., Min, J., Guo, R.-T.,* Chen, C.-C.* Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin. International Journal of Biological Macromolecules, 2022, 222: 421-428.
13. Wu, L., # An, J., # Jing, X., # Chen, C.-C., Dai, L., Liu, W.,* Guo, R.-T.,* Nie, Y.* Molecular insights into the regioselectivity of the Fe(II)/2-ketoglutarate-dependent dioxygenase-catalyzed C-H hydroxylation of amino acids. ACS Catalysis, 2022, 12(19): 11586-11596.
14. Zhang, L., # Zhang, X., # Min, J., # Liu, B., Huang, J.-W., Yang, Y., Liu, W., Dai, L., Yang, Y., Chen, C.-C.,* Guo, R.-T.* Structural insights to a bi-functional isoprenyl dihposphate synthase that can catalyze head-to-tail and head-to-middle condensation. International Journal of Biological Macromolecules ,2022, 214: 492-499.
15. Li, H., # Yang, Y., # Hu, Y., # Chen, C.-C., Huang, J.-W., Min, J., Dai, L.,* Guo, R.-T.* Structural analysis and engineering of aldo-keto reductase from glyphosate-resistant Echinochloa colona. Journal of Hazardous Materials, 2022, 436: 129191.
16. Malwal, S. R., # Shang, N., #Liu, W., Li, X., Zhang, L., Chen, C.-C., Guo, R.-T.,* Oldfield, E.* A structural and bioinformatics investigation of a fungal squanlene synthase and comparisons with other membrane proteins. ACS Omega, 2022, 7(26): 22601-22612.
17. Wang, T., # Yang, Y., # He, M., Liu, M., Huang, J.-W., Min, J., Chen, C.-C., Liu, Y.,* Zhang, L.,* Guo, R.-T.* Structural insights into the cyclization of unusual brasilane-type sesquiterpenes. International Journal of Biological Macromolecules , 2022, 209: 1784-1791.
18. Dai, L., # Zhang, X., # Hu, Y., # Shen, J., Zhang, Q., Zhang, L., Min, J., Chen, C.-C., Liu, Y.,* Huang, J.-W.,* Guo, R.-T.* Structural and functional insights into a nonheme iron- and α-ketoglutarate-dependent halogenase that catalyzes chlorination of nucleotide substrates. Applied and Environmental Microbiology, 2022, 88(9): e0249721.
19. Zeng, W., # Li, X., # Yang, Y., # Min, J., # Huang, J.-W., Liu, W., Niu, D., Yang, X., Han, X., Zhang, L., Dai, L., Chen, C.-C.,* Guo, R.-T.* Substrate-binding mode of a thermophilic PET hydrolase and engineering the enzyme to enhance the hydrolytic efficacy. ACS Catalysis, 2022, 12(5): 3033-3040.
20. Chen, C.-C., # Dai, M., # Zhang, L., # Zhao, J., Zeng, W., Shi, M., Huang, J.-W., Liu, W., Guo, R.-T.,* Li, A.* Molecular basis for a toluene monooxygenase to govern substrate selectivity. ACS Catalysis, 2022, 12(5): 2831-2839.
21. Mao, Z., # Xiao, H., # Shen, P., #Yang,Y., # Xue, J., Yang, Y., Shang, Y., Zhang, L., Li, X., Zhang, Y., Du, Y., Chen, C.-C., Guo, R.-T.,* Zhang, Y.* KRAS(G12D) can be targeted by potent inhibitors via formation of salt bridge. Cell Discovery ,2022, 8(1): 5.
22. Hu, Y. #, Li, H., # Min, J., # Yu, Y., Liu, W., Huang, J.-W., Zhang, L., Yang, Y., Dai, L.*, Chen, C.-C.,* Guo, R.-T.* Crystal structure and biochemical analysis of the specialized deoxynivalenol–detoxifying glyoxalase SPG from Gossypium hirsutum. International Journal of Biological Macromolecules, 2022, 200: 388-396.
23. 陈纯琪, 韩旭, 刘卫东, 马立新, 刘珂*, 郭瑞庭 *. 基于结构改造来源于大阪伊德氏杆菌 201-F6 的PET水解酶. 生物工程学报,2021, 37(9): 3268-3275.
24. Dai, L., # Chang, Z., # Yang, J., Liu,W., Yang, Y., Chen, C.-C., Zhang, L., Huang, J.-W.,* Sun, Y.,* Guo, R.-T.* Structural investigation of a thermostable 1,2-β-mannobiose phosphorylase from Thermoanaerobacter sp . X-514. Biochemical and Biophysical Research Communications, 2021, 579: 54-61.
25. Dai, L., # Qu, Y., # Hu, Y., # Min, J., Yu, X., Chen, C.-C., Huang, J.-W.,* Guo, R.-T.* Catalytically inactive lytic polysaccharide monooxygenase PcAA14A enhances the enzyme-mediated hydrolysis of polyethylene terephthalate. International Journal of Biological Macromolecules ,2021, 190: 456-462.
26. Dai, L., # Qu, Y., #Huang, J.-W., Hu, Y., Hu, H., Li, S., Chen, C.-C.,* Guo, R.-T.* Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I from Trichoderma reesei. Journal of Biotechnology, 2021, 334: 47-50.
27. Liu, W., # Ma, C., # Liu, W., #Zheng, Y., Chen, C.-C., Liang, A., Luo, X., Li, Z., Ma, W., Song, Y.,* Guo, R.-T.,* Zhang, T.* Functional and structural investigation of a novel β-mannanase BaMan113A from Bacillus sp . N16-5. International Journal of Biological Macromolecules, 2021, 182: 899-909.
28. Chen, C.-C., # Han, X., # Li, X., # Jiang, P., Niu, D., Ma, L., Liu, W., Li, S., Qu, Y., Hu, H., Min, J., Yang, Y., Zhang, L., Zeng, W., Huang, J.-W.,* Dai, L.,* Guo, R.-T.* General features to enhance enzymatic activity of poly(ethylene terephthalate) hydrolysis. Nature Catalysis, 2021, 4(5): 425-430. (Highlighted by ChemistryViews, 科技日报 , 中国科学报 )
29. Dai, L., Hu, Y., Chen, C.-C., Ma, L.,* Guo, R.-T.* Flavonoid C-glycosyltransferases: function, evolutionary relationship, catalytic mechanism and protein engineering. ChemBioEng Reviews, 2021, 21(1): 15-26.
30. Chen, C.-C., # Yu, X., # Kuo, C.-J., # Min, J., Chen, S., Ma, L.,* Liu, K.,* Guo, R.-T.* Overview of antiviral drug candidates targeting coronaviral 3C-like main proteases. FEBS Journal, 2021, 228(17): 5089-5121. (Review, https://doi.org/10.1111/febs.15696 )
31. Chen, C.-C., Min, J., Zhang, L., Yang, Y., Yu, X.,* Guo, R.-T.* Advanced understanding of the electron transfer pathway of cytochrome P450s. ChemBioChem, 2021, 22(8): 1317-1328. (Review, Cover story)
32. Chen, C.-C., # Malwal, S. R., # Han, X., # Liu, W., Ma, L., Zhai, C., Dai, L., Huang, J.-W., Shillo, A., Desai, J., Ma, X., Zhang, Y., Guo, R.-T.,* Oldfield, E.* Terpene cyclases and prenyltransferases: structures and mechanisms of action. ACS Catalysis, 2021, 11(1): 290-303. ( Cover story)
33. Chen, C.-C., # Jian Min, J., # Zhang, L., Yang, Y., Yu, X.,* Guo, R.-T.* Advanced understanding of electron transfer pathway of cytochrome P450s. ChemBioChem,2021, 22(8): 1317-1328. (Review, Cover story)
34. Chen, C.-C., Xue, J., Peng, W., Wang, B., Zhang, L., Liu, W., Ko, T.-P., Huang, J.-W., Zhou, S., Min, J., Ma, L., Dai, L.,* Guo, R.-T.,* Yu, X.* Structural insights into thebaine synthase 2 catalysis. Biochemical and Biophysical Research Communications, 2020, 529(2):156-161.
35. Zhang, L., # Xie, Z., # Liu, Z., # Zhou, S., Ma, L., Liu, W., Huang, J.-W., Ko, T.-P., Li, X., Hu, Y., Min, J., Yu, X., Guo, R.-T.,* Chen, C.-C.* Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase. Nature Communications, 2020, 11(1): 2676. (Highlighted by C&EN, ChemistryViews, 科技日报 , 中国科学报 )
36. Chen, C.-C.,* Zhang, L., Yu, X., Ma, L., Ko, T.-P., Guo, R.-T.* Versatile cis-isoprenyl diphosphate synthase superfamily members in catalyzing carbon–carbon bond formation. ACS Catalysis, 2020, 10(8): 4717-4725. (Review, ACS Editors’ Choice, Cover story)
37. Chen, C.-C., Dai, L., Ma, L.,* Guo, R.-T.* Enzymatic degradation of plant biomass and synthetic polymers. Nature Reviews Chemistry, 2020, 4(3): 114-126. (Review, Cover story)
38. Song, M., Zhang, X., Liu, W.,* Feng, J.,* Cui, Y., Yao, P., Wang, M., Guo, R.-T., Wu, Q., Zhu, D.* 2,3-dihydroxybenzoic acid decarboxylase from Fusarium oxysporum: crystal structures and substrate recognition mechanism. ChemBioChem, 2021, 21(20): 2950-2956.
39. Huang, J.-W., # Niu, D., # Liu, K., # Wang, Q., Ma, L., Chen, C.-C., Zhang, L., Liu, W., Zhou, S., Min, J., Wu, S., Yang, Y.,* Guo, R.-T.* Structure basis of non-structural protein pA151R from African Swine Fever Virus. Biochemical and Biophysical Research Communications,2020, 532(1): 108-113.
40. Liu, Y., Lee, C., Li, F., Trček, J., Bähre, H., Guo, R.-T., Chen, C.-C., Chernobrovkin, A., Zubarev, R., Römling, U. A cyclic di-GMP network is present in gram-positive streptococcus and gram-negative proteus species. ACS Infectious Diseases, 2020, 6(10): 2672-2687.
41. Xiao, X., Elsayed, S. S., Wu, C., van der Heul, H. U., Metsä-Ketelä, M., Du, C., Prota, A. E., Chen, C.-C., Liu, W., Guo, R.-T., Abrahams, J. P., van Wezel, G. P. Functional and structural insights into a novel promiscuous ketoreductase of the lugdunomycin biosynthetic pathway. ACS Chemical Biology, 2020, 15(9): 2529-2538.
42. Zhou, S., Ko, T.-P.,* Huang, J.-W., Liu, W., Zheng, Y., Wu, S., Wang, Q., Xie, Z., Liu, Z., Chen, C.-C.,* Guo, R.-T.* Structure of a gut microbial diltiazem-metabolizing enzyme suggests possible substrate binding mode. Biochemical and Biophysical Research Communications,2020, 527(3): 799-804.
43. Chen, X., Zhang, H., Maria-S., Miguel A., Liu, W., Li, J., Feng, J., Liu, X., Osuna S.,* Guo, R.-T., Wu, Q.,* Zhu, D.,* Ma, Y. Efficient reductive desymmetrization of bulky 1, 3-cyclodiketones enabled by structure-guided directed evolution of a carbonyl reductase. Nature Catalysis, 2019, 2(10): 931-941.
44. Yang, Y., Li, L., Yuan, L., Zhou, X., Duan, J., Xiao, H., Cai, N., Han, S., Ma, X., Liu, W., Chen, C.-C., Wang, L., Li, X., Chen, J., Kang, N., Chen, J., Shen, Z., Malwal, S. R., Liu, W., Shi, Y., Oldfield, E., Guo, R.-T.,* Zhang, Y.* A structural change in butyrophilin upon phosphoantigen binding underlies phosphoantigen-mediated Vgamma9Vdelta2 T cell activation. Immunity,2019, 50(4): 1043-1053.
45. Sun, H., Ko, T.-P., Liu, W., Liu, W., Zheng, Y., Chen, C.-C.,* Guo, R.-T.* Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate. Biochemical and Biophysical Research Communications,2019, 521(1): 31-36.
46. Kuo, C.-J., Gao, J., Huang, J.-W., Ko, T.-P., Zhai, C., Ma, L., Liu, W., Dai, L., Chang, Y.-F., Chen, T.-H., Hu, Y., Yu, X., Guo, R.-T.,* Chen, C.-C.* Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis. Biochimica et Biophysica Acta. General subjects, 2019, 1863(9): 1351-1359.
47. Tang, X., # Xue, J., # Yang, Y., Ko, T.-P., Chen, C.-Y., Dai, L., Guo, R.-T., Zhang, Y.,* Chen C. C.* Structural insights into the calcium dependence of Stig cyclases. RSC Advances,2019, 9(23): 13182.
48. Ma, J., Ko, T.-P., Yu, X., Zhang, L., Ma, L., Zhai, C., Guo, R.-T., Liu, W., Li, H., Chen, C.-C.* Structural insights to heterodimeric cis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1. Biochemical and Biophysical Research Communications, 2019, 515(4): 621-626.
49. Zhang, L., Ko, T.-P., Malwal S. R., Liu W., Zhou S., Oldfield E., Guo R.-T.,* Chen, C.-C.* Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism. Biochemical and Biophysical Research Communications, 2019, 511(4): 800-805.
50. Chang, Z., Ansbacher, T., Zhang, L., Yang, Y., Ko, T.-P., Zhang, G., Liu, W., Huang, J.-W., Dai, L., Guo, R.-T., Major, D. T.,* Chen, C.-C.* Crystal structure of LepI, a multifunctional SAM-dependent enzyme which catalyzes pericyclic reactions in leporin biosynthesis. Organic Biomolecular Chemistry, 2019, 17(8): 2070-2076. (Cover story)
51. Malwal, S. R., Chen, L., Hicks, H., Qu, F., Liu, W., Shillo, A., Law, W. X., Zhang, J., Chandnani, N., Han, X., Zheng, Y., Chen, C.-C., Guo, R.-T. , AbdelKhalek, A., Seleem, M. N., Oldfield, E. Discovery of lipophilic bisphosphonates that target bacterial cell wall and quinone biosynthesis. Journal of M edicinal C hemistry,2019, 62(5): 2564-2581.
52. Zhang, M., Yu, X.-W., Xu, Y., Guo, R.-T., Swapna, G. V. T, Szyperski, T., Hunt, J. F., Montelione, G. T. Structural basis by which the N-terminal polypeptide segment of Rhizopus chinensis lipase regulates its substrate binding affinity. Biochemistry, 2019, 58(38): 3943-3954.
53. Chen, C.-C., # Hu, X., # Tang, X., # Yang, Y., # Ko, T.-P., Gao, J., Zheng, Y., Huang, J.-W., Yu, Z., Li, L., Han, S., Cai, N., Zhang, Y.,* Liu, W.,* Guo, R.-T. * The crystal structure of a class of cyclases that catalyze the Cope rearrangement. Angewandte Chemie International Edition, 2018, 57(46): 15060-15064. (Cover story)
54. Gao, J., # Ko, T.-P., # Chen, L., # Malwal, S. R., Zhang, J., Hu, X., Qu, F., Liu, W., Huang, J.-W., Cheng, Y.-S., Chen, C.-C., Yang, Y., Zhang, Y., Oldfield, E., Guo, R.-T. * “Head-to-middle” and “head-to-tail” cis-prenyl transferases: structure of isosesquilavandulyl diphosphate synthase. Angewandte Chemie International Edition, 2018, 57(3): 683-687. (Cover story)
55. Chen, C.-C., # Han, X., # Ko, T.-P., Liu, W.,* Guo, R.-T. * Structural studies reveal the molecular mechanism of PETase. FEBS Journal, 2018, 285(20): 3717-3723.
56. You, S., Chen, C.-C., Tu, T., Wang, X., Ma, R., Cai, H.-Y., Guo, R.-T. ,* Luo, H.-Y.,* Yao, B.* Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis. Biotechnol Biofuels, 2018, 11:159.
57. Zheng, Y., # Liu, W., # Chen C. C., # Hu, X., Liu, W., Ko, T.-P., Tang, X., Wei, H., Huang, J.-W., Guo, R.-T. * Crystal structure of a mycoestrogen-detoxifying lactonase from Rhinocladiella mackenziei: Molecular insight into ZHD substrate selectivity. ACS Catalysis. 2018, 8(5): 4294-4298.
58. Malwal, S. R., # Gao, J., # Hu, X., # Yang, Y., # Liu, W., Huang, J.-W., Ko, T.-P., Li, L., Chen, C.-C., O’Dowd, B., Khade, R. L., Zhang, Y., Zhang, Y., Oldfield, E., Guo, R.-T. * Catalytic role of conserved asparagine, glutamine, serine, and tyrosine residues in isoprenoid biosynthesis enzymes. ACS Catalysis. 2018, 8(5): 4299-4312.
59. Lee, S. M.-Y., Yip, T., Yan, S., Jin, D., Wei, H., Guo, R.-T., Peiris, JS Malik.* Recognition of double-stranded RNA and regulation of interferon pathway by toll-like receptor 10. Frontiers in Immunology .2018, 9: 516.
60. Wang, J., # Chen, C.-C., # Yang, Y., # Liu, W., Ko, T.-P., Shang, N., Hu, X., Xie, Y., Huang, J.-W., Zhang, Y.,* Guo, R.-T. * Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis. Biochemical and B iophysical R esearch C ommunications , 2018, 495(2): 1782-1788.
61. Han, X., # Liu, W., # Huang, J.-W., # Ma, J., Zheng, Y., Ko, T.-P., Xu, L., Cheng, Y.-S., Chen, C.-C., Guo, R.-T. * Structural insight into catalytic mechanism of PET hydrolase. Nature Communications, 2017, 8(1): 2106.
62. Gao, J., # Huang, J.-W., # Li, Q., # Liu, W., Ko, T.-P., Zheng, Y., Xiao, X., Kuo, C.-J., Chen, C.-C.,* Guo, R.-T. * Characterization and crystal structure of a thermostable glycoside hydrolase family 45 1,4-β-endoglucanase from Thielavia terrestris. Enzyme and Microbial Technology, 2017,99: 32-37.
63. Schwalen, C. J., Feng, X., Liu, W., O-Dowd, B., Ko, T.-P., Shin, C. J., Guo, R.-T. , Mitchell, D. A.,* Oldfield, E.* Head-to-head prenyl synthases in pathogenic bacteria. ChemBioChem, 2017, 18(11): 985-991.
64. Hui, R., # Hu, X., # Liu, W., Zheng, Y., Chen, Y., Guo, R.-T. , Jin, J.,* Chen, C.-C.* Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana. Acta C rystallographica. S ection F, S tructural B iology C ommunications , 2017, 73: 515-519.
65. Zhang, L., # Chen, C.-C., # Ko, T.-P., # Huang, J.-W., Zheng, Y., Liu, W., Wang, I., Malwal, S. R., Feng, X., Wang, K., Huang, C. C., Hsu, S.-T. D., Wang, A. H.-J., Oldfield, E.,* and Guo, R.-T. * Moenomycin biosynthesis: structure and mechanism of action of the prenyl transferase MoeN5. Angewandte Chemie International Edition, 2016, 55(15) : 4716-4720.
66. Xu, Z., # Liu, W., # Chen, C.-C., # Li Q., Huang, J.-W., Ko, T.-P., Liu, G., Liu, W., Peng, W., Cheng, Y.-S., Chen, Y., Jin, J., Li, H.,* Zheng, Y.,* Guo, R.-T. * Enhanced α‑zearalenol hydrolyzing activity of a mycoestrogen detoxifying lactonase by structure-based engineering. ACS Catalysis, 2016, 6(11): 7657-7663. (Cover story)
67. Liu, M., # Chen, C.-C., # Chen, Lu., # Xiao, X., Zheng, Y.,Huang, J.-W., Liu, W., Ko, T.-P., Cheng, Y.-S., Feng, X., Oldfield, E.,* Guo, R.-T. ,* and Ma, Y.* Structure and function of a “head-to-middle” prenyltransferase: lavandulyl diphosphate synthase. Angewandte Chemie International Edition, 2016, 55(15): 4721-4724. (Cover story)
68. Wang, Y., Desai, J., Zhang, Y., Malwal, S. R., Shin, C. J., Feng, X., Sun, H., Liu, G., Guo, R.-T. , Oldfield, E.* Bacterial cell growth inhibitors targeting undecaprenyl diphosphate synthase and undecaprenyl diphosphate phosphatase. ChemMedChem, 2016, 11(20): 2311-2319.
69. Yan, J., # Liu, W., # Li, Y., Lai, H.-L., Zheng, Y., Huang, J.-W., Chen, C.-C., Chen, Y., Jin, J., Li, H.,* Guo, R.-T. * Functional and structural analysis of Pichia pastoris expressed Aspergillus niger 1,4-β-endoglucanase. Biochemical and B iophysical R esearch C ommunications , 2016, 475(1): 8-12.
70. Desai, J., Liu, Y.-L., Wei, H., Liu, W., Ko, T.-P., Guo, R.-T. , Oldfield, E.* Structure, function, and inhibition of Staphylococcus aureus heptaprenyl diphosphate synthase. ChemMedChem, 2016, 11(17): 1915-1923.
71. Huang, J.-W., # Liu, W., # Lai, H.-L., Cheng, Y.-S., Zheng, Y., Li, Q., Sun, H., Kuo, C.-J., Guo, R.-T. ,* Chen, C.-C.* Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50. Biochemical and B iophysical R esearch C ommunications , 2016, 478(2): 565-572.
72. Yang, Y., # Ko, T.-P., # Chen, C.-C., # Huang, G., Zheng, Y., Liu, W., Wang, I., Ho, M., Hsu, S., O'Dowd, B., Huff, H., Huang, C.-H., Docampo, R., Oldfield, E.,* Guo, R.-T. * Structures of trypanosome vacuolar soluble pyrophosphatases: anti-parasitic drug targets. ACS Chemical Biology, 2016, 11(5): 1362-1371.
73. Zheng, Y., # Li, Y., # Liu, W., # Chen, C.-C., Ko, T.-P., He, M., Xu, Z., Liu, M., Luo, H., Guo, R.-T., * Yao, B,* Ma, Y.* Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-β-1, 4-xylanase. Journal of Structural Biology, 2016, 193(3): 206-211. (Cover story)
74. Liu, G., Li, Q., Shang, N., Huang, J.-W., Ko, T.-P., Liu, W., Zheng, Y., Han, X., Chen, Y., Chen, C.-C., Jin, J., Guo, R.-T. * Functional and structural analyses of a 1,4-β-endoglucanase from Ganoderma lucidum. Enzyme and Microbial Technology, 2016, 86: 67-74.
75. Hu, Y., # Liu, W., # Malwal, S. R.,Zheng, Y., Feng, X., Ko, T.-P., Chen, C.-C., Xu, Z., Liu, M., Han, X., Gao, J., Oldfield, E.,* Guo, R.-T. * Structures of iridoid synthase from Cantharanthus roseus with bound NAD (+), NADPH, or NAD (+) /10-oxogeranial: reaction mechanisms. Angewandte Chemie International Edition, 2015, 54(51):15478-15482. (Cover story)
76. Cheng, Y.-S., Chen, C.-C., Huang, J.-W., Ko, T.-P., Huang, Z., Guo, R.-T. * Improving the catalytic performance of a GH11 xylanase by rational protein engineering. Applied Microbiology and Biotechnology, 2015, 99(22): 9503-9510.
77. Zheng, Y., Chen, C.-C., Ko, T.-P., Xiao, X., Yang, Y., Huang, C.-H., Qian, G., Shao, W.,* Guo, R.-T. * Crystal structures of S-adenosyl homocysteine hydrolase from the thermophilic bacterium Thermotoga maritima. Journal of Structural Biology, 2015, 190(2): 135-142. (Cover story)
78. Guo, R.-T. * Green Enzymes. ChemBioEng Reviews, 2015, 2(2): 75. (Invited Editorial)
79. Chen, C.-C., Cheng, K. J., Ko, T.-P., Guo, R.-T. *Current progresses in phytase research: three-dimensional structure and protein engineering. ChemBioEng Reviews, 2015, 2(2): 76-86. (Cover story)
80. Chen, C.-C., Ko, T.-P., Huang, J.-W., Guo, R.-T. *Heat- and alkaline-stable xylanases: applications, protein structure and engineering. ChemBioEng Reviews, 2015, 2(2): 95-106.
81. Chan, H.-C., # Ko, T.-P., # Huang, C.-H., Guo, R.-T. *Minireview: A comeback of Hg-derivatives in protein crystallography with Cys-modification. ChemBioEng Reviews, 2015, 2(2): 133-140.
82. Liu, W., Guo, R.-T. , Chen, X., Li, Z., Gao, X., Huang, C.-H., Wu, Q., Feng, J., Zhu, D.* Structural analysis reveals the substrate-binding mechanism for the expanded substrate specificity of mutant meso-diaminopimelate dehydrogenase. ChemBioChem, 2015, 16(6): 924-929. (Cover story)
83. Chen, Y., Huang, J.-W., Chen, C.-C., Lai, H.-L., Jin, J., Guo, R.-T. * Crystallization and preliminary X-ray diffraction analysis of an endo-1, 4-beta-D-glucanase from Aspergillus aculeatus F-50. Acta Crystallographica. Section F, Structural Biology Communications, 2015, 71: 397-400.
84. Zhu, W., Wang, Y., Li, K., Gao, J., Huang, C.-H., Chen, C.-C., Ko, T.-P., Zhang, Y., Guo, R.-T., Oldfield, E.* Antibacterial drug leads: DNA and enzyme multi-targeting. Journal of M edicinal C hemistry , 2015, 58(3): 1215-1227.
85. Zheng, Y., # Ko, T.-P., # Yang, Y., Shao, W., * Guo, R.-T.* Binding mode of the oxidized alpha-anomer of NAD + to RSP, a Rex-family repressor. Biochemical and B iophysical R esearch C ommunications , 2015, 456(3): 733-736.
86. Yuan, S.-F., Wu, T.-H., Lee, H.-L., Hsieh, H.-Y., Lin, W.-L., Yang, B., Chang, C.-K., Li, Q., Gao, J., Huang, C.-H., Ho, M.-C.,* Guo, R.-T.,* Liang, P.-H.* Biochemical characterization and structural analysis of a bi-functional cellulase/xylanase from Clostridium thermocellum. The Journal of B iological C hemistry , 2015, 290(9): 5739-5748.
87. Chen, C.-C., # Huang, J.-W., # Zhao, P., Ko, T.-P., Huang, C.-H., Chan, H.-C., Huang, Z., Liu, W., Cheng, Y.-S., Liu, J.-R., Guo, R.-T.* Structural analyses and yeast production of the β-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum. Enzyme and Microbial Technology , 2015, 71: 1-7.
88. Veiga-Santos, P., Li, K., Lameira, L., de Carvalho, T. M., Huang, G., Galizzi, M., Shang, N., Li, Q., Gonzalez-Pacanowska, D., Hernandez-Rodriguez, V., Benaim, G., Guo, R.-T., Urbina, J. A., Docampo, R., de Souza, W., Oldfield, E.* SQ109, a new drug lead for Chagas disease. Antimicrobial A gents and C hemotherapy , 2015, 59(4): 1950-1961.
89. Han, X., # Chen, C.-C., # Kuo, C.-J., Huang, C.-H., Zheng, Y., Ko, T.-P., Zhu, Z., Feng, X., Wang, K., Oldfield, E., Wang, A. H.-J., Liang, P.-H., Guo, R.-T.,* Ma, Y.* Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from Escherichia coli reveal the catalytic and chain-length determining mechanisms. Proteins, 2015, 83(1): 37-45.
90. Chan, H.-C., # Feng, X., # Ko, T.-P. #, Huang, C.-H., Hu, Y., Zheng, Y., Bogue, S., Nakano, C., Hoshino, T., Zhang, L., Lv, P., Liu, W., Crick, D. C., Liang, P.-H., Wang, A. H.-J., Oldfield, E.,* Guo, R.-T. * Structure and inhibition of tuberculosinol synthase and decaprenyl diphosphate synthase from Mycobacterium tuberculosis. Journal of the A merican Chemical Society , 2014, 136(7): 2892-2896.
91. Liu, W., # Feng, X., # Zheng, Y., # Huang, C.-H., Nakano, C., Hoshino, T., Bogue, S., Ko, T.-P., Chen, C.-C., Cui, Y., Li, J., Wang, I., Hsu, S.-T. D., Oldfield, E.,* Guo, R.-T.* Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum. Scientific R eports , 2014, 4: 6214.
92. Cheng, Y.-S., # Chen, C.-C., # Huang, C.-H., Ko, T.-P., Luo, W., Huang, J.-W., Liu, J.-R., Guo, R.-T.* Structural analysis of a glycoside hydrolase family 11 xylanase from Neocallimastix patriciarum: insights into the molecular basis of a thermophilic enzyme. The Journal of B iological C hemistry , 2014, 289(16): 11020-11028.
93. Shang, N., # Li, Q., # Ko, T.-P., # Chan, H.-C., Li, J., Zheng, Y., Huang, C.-H., Ren, F., Chen, C.-C., Zhu, Z., Galizzi, M., Li, Z. H., Rodrigues-Poveda, C. A., Gonzalez-Pacanowska, D., Veiga-Santos, P., de Carvalho, T. M., de Souza, W., Urbina, J. A., Wang, A. H.-J., Docampo, R., Li, K., Liu, Y.-L., Oldfield, E.,* Guo, R.-T.* Squalene synthase as a target for Chagas disease therapeutics. PLoS Pathogens, 2014, 10(5): e1004114.
94. Zheng, Y., # Ko, T.-P., # Sun, H., Huang, C.-H., Pei, J., Qiu, R., Wang, A. H.-J., Wiegel, J., Shao, W.,* Guo, R.-T.* Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes. Journal of S tructural B iology , 2014, 188(3): 195-204. (Cover story)
95. Peng, W., # Ko, T.-P., # Yang, Y., Zheng, Y., Chen, C.-C., Zhu, Z., Huang, C.-H., Zeng, Y.-F., Huang, J.-W., Wang, A. H.-J.-J., Liu, J.-R.,* Guo, R.-T.* Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea. RSC Advances, 2014, 4(107): 62321-62325.
96. Zhu, Z., # He, M., # Huang, C.-H., Ko, T.-P., Zeng, Y.-F., Huang, Y.-N., Jia, S., Lu, F., Liu, J.-R., Guo, R.-T.* Crystallization and preliminary X-ray diffraction analysis of a novel beta-L-arabinofuranosidase (HypBA1) from Bifidobacterium longum. Acta C rystallographica. Section F, Structural B iology C ommunications , 2014, 70: 636-638.
97. Huang, C.-H., Zhu, Z., Cheng, Y.-S., Chan, H.-C., Ko, T.-P., Chen, C.-C., Wang, I., Ho, M.-R., Hsu, S.-T. D. -D., Zeng, Y.-F., Huang, Y.-N., Liu, J.-R.,* Guo, R.-T.* Structure and catalytic mechanism of a glycoside hydrolase family-127β-L-arabinofuranosidase (HypBA1). J Bioprocess Biotech, 2014, 4(6): 171.
98. Zhang, L., # Zhao, P., # Chen, C.-C., Huang, C.-H., Ko, T.-P., Zheng, Y., Guo, R.-T.* Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase from Clostridium thermocellum. Acta C rystallographica. Section F, Structural B iology C ommunications , 2014, 70: 946-948.
99. Yang, Y., # Ko, T.-P., # Liu, L., Li, J., Huang, C.-H., Chen, J., Guo, R.-T.,* Du, G.* Roles of tryptophan residue and disulfide bond in the variable lid region of oxidized polyvinyl alcohol hydrolase. Biochemical and B iophysical R esearch C ommunications , 2014, 452(3): 509-514.
100. Yang, Y., # Ko, T.-P., # Liu, L., Li, J., Huang, C.-H., Chan, H.-C., Ren, F., Jia, D., Wang, A. H.-J., Guo, R.-T.,* Chen, J.,* Du, G.* Structural insights into enzymatic degradation of oxidized polyvinyl alcohol. Chem B io C hem : a European J ournal of C hemical B iology , 2014, 15(13): 1882-1886. (Cover story)
101. Xu, J., # Ren, F., # Huang, C.-H., Zheng, Y., Zhen, J., Sun, H., Ko, T.-P., He, M., Chen, C.-C., Chan, H.-C., Guo, R.-T.,* Song, H.,* Ma, Y.* Functional and structural studies of pullulanase from Anoxybacillus sp. LM18-11. Proteins, 2014, 82(9): 1685-1693. (Cover story)
102. Wu, T.-H., # Chen, C.-C., # Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Lai, H.-L., Huang, T.-Y., Liu, J.-R.,* Guo, R.-T.* Improving specific activity and thermostability of Escherichia coli phytase by structure-based rational design. Journal of B iotechnology , 2014, 175: 1-6.
103. Wang, S., Nie, Y., Xu, Y.,* Ko, T.-P., Huang, C.-H., Chan, H.-C., Guo, R.-T.,* Xiao, R. Crystallization and preliminary X-ray diffraction analysis of (R)-carbonyl reductase from Candida parapsilosis. Acta C rystallographica. Section F, Structural B iology C ommunications , 2014, 70: 800-802.
104. Wang, S., Nie, Y., Xu, Y.,* Zhang, R., Ko, T.-P., Huang, C.-H., Chan, H.-C., Guo, R.-T.,* Xiao, R. Unconserved substrate-binding sites direct the stereoselectivity of medium-chain alcohol dehydrogenase. Chemical C ommunications , 2014, 50(58): 7770-7772. (Cover story)
105. Tseng, C.-W., # Liao, C.-Y., # Sun, Y., Peng, C.-C., Tzen, J. T., Guo, R.-T.,* Liu, J.-R.* Immobilization of Clostridium cellulolyticum D-psicose 3-epimerase on artificial oil bodies. Journal of A gricultural and F ood C hemistry , 2014, 62(28): 6771-6776.
106. Lv, P., # Zhang, L., # Luo, H., Chen, C.-C., Huang, C.-H., Peng, W., Wang, K., Ko, T.-P., Zheng, Y., Zhang, J., Yao, B.,* Guo, R.-T.* Preliminary X-ray diffraction analysis of thermostable beta-1,4-xylanase from Streptomyces sp. S9. Acta C rystallographica. Section F, Structural B iology C ommunications , 2014, 70: 105-107.
107. Liu, W., Li, Z., Huang, C.-H., Guo, R.-T., Zhao, L., Zhang, D., Chen, X., Wu, Q., Zhu, D.* Structural and mutational studies on the unusual substrate specificity of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum. Chem B io C hem , 2014, 15(2): 217-222. (Cover story)
108. Li, K., Schurig-Briccio, L. A., Feng, X., Upadhyay, A., Pujari, V., Lechartier, B., Fontes, F. L., Yang, H., Rao, G., Zhu, W., Gulati, A., No, J.-H., Cintra, G., Bogue, S., Liu, Y.-L., Molohon, K., Orlean, P., Mitchell, D. A., Freitas-Junior, L., Ren, F., Sun, H., Jiang, T., Li, Y., Guo, R.-T., Cole, S. T., Gennis, R. B., Crick, D. C., Oldfield, E.* Multitarget drug discovery for tuberculosis and other infectious diseases. Journal of M edicinal C hemistry , 2014, 57(7): 3126-3139.
109. Huang, J.-W., # Chen, C.-C., # Huang, C.-H., Huang, T.-Y., Wu, T.-H., Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Liu, J.-R.,* Guo, R.-T.* Improving the specific activity of beta-mannanase from Aspergillus niger BK01 by structure-based rational design. Biochimica et B iophysica A cta , 2014, 1844(3): 663-669. (Cover story)
110. He, M., # Zheng, Y., # Huang, C.-H., Qian, G., Xiao, X., Ko, T.-P., Shao, W., Guo, R.-T.* Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima. Acta C rystallographica. Section F, Structural B iology C ommunications , 2014, 70: 1563-1565.
111. Feng, X., # Hu, Y., # Zheng, Y., # Zhu, W., Li, K., Huang, C.-H., Ko, T.-P., Ren, F., Chan, H.-C., Nega, M., Bogue, S., Lopez, D., Kolter, R., Gotz, F., Guo, R.-T.,* Oldfield, E.* Structural and functional analysis of Bacillus subtilis YisP reveals a role of its product in biofilm production. Chemistry & B iology , 2014, 21(11): 1557-1563.
112. Cheng, Y.-S., # Huang, C.-H., # Chen, C.-C., Huang, T.-Y., Ko, T.-P., Huang, J.-W., Wu, T.-H., Liu, J.-R.,* Guo, R.-T.* Structural and mutagenetic analyses of a 1,3-1,4-beta-glucanase from Paecilomyces thermophila. Biochimica et B iophysica A cta , 2014, 1844(2): 366-373. (Cover story)
113. Chen, C.-C., # Luo, H., # Han, X., # Lv, P., Ko, T.-P., Peng, W., Huang, C.-H., Wang, K., Gao, J., Zheng, Y., Yang, Y., Zhang, J., Yao, B.,* Guo, R.-T.* Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability. Journal of B iotechnology , 2014, 189: 175-182.
114. Bai, W., Zhou, C., Xue, Y., Huang, C.-H., Guo, R.-T., Ma, Y.* Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp. SN5 and improvement of its thermal performance by introducing arginines substitutions. Biotechnology L etters , 2014, 36(7): 1495-1501.
115. Ren, F., # Feng, X., # Ko, T.-P., # Huang, C.-H., Hu, Y., Chan, H.-C., Liu, Y.-L., Wang, K., Chen, C.-C., Pang, X., He, M., Li, Y., Oldfield, E.,* Guo, R.-T.* Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus. Chem B io C hem , 2013, 14(2): 195-199. (Cover story)
116. Luo, W., # Huang, J.-W., # Huang, C.-H., Huang, T.-Y., Chan, H.-C., Liu, J.-R., Guo, R.-T., Chen, C.-C.* Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01. Acta C rystallographica. Section F, Structural B iology and C rystallization C ommunications , 2013, 69: 1100-1102.
117. Li, X., # Han, X., # Ko, T.-P., Chen, C.-C., Zhu, Z., Hua, E., Guo, R.-T., Huang, C.-H.* Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli. Acta crystallographica. Section F, Structural B iology and C rystallization C ommunications , 2013, 69: 328-331.
118. Jiang, T., # Chan, H.-C., # Huang, C.-H., Ko, T.-P., Huang, T.-Y., Liu, J.-R., Guo, R.-T.* Substrate binding to a GH131 beta-glucanase catalytic domain from Podospora anserina. Biochemical and B iophysical R esearch C ommunications , 2013, 438(1): 193-197.
119. Hu, Y., Jia, S., Ren, F., Huang, C.-H., Ko, T.-P., Mitchell, D. A., Guo, R.-T., Zheng, Y.* Crystallization and preliminary X-ray diffraction analysis of YisP protein from Bacillus subtilis subsp. subtilis strain 168. Acta C rystallographica. Section F, Structural B iology and C rystallization C ommunications , 2013, 69: 77-79.
120. Han, X., # Gao, J., # Shang, N., # Huang, C.-H., Ko, T.-P., Chen, C.-C., Chan, H.-C., Cheng, Y.-S., Zhu, Z., Wiegel, J., Luo, W.,* Guo, R.-T.,* Ma, Y.* Structural and functional analyses of catalytic domain of GH10 xylanase from Thermoanaerobacterium saccharolyticum JW/SL-YS485. Proteins, 2013, 81(7): 1256-1265. (Cover story)
121. Chang, T.-H., # Chang, S. J., # Hsieh, F. L., Ko, T.-P., Lin, C.-T., Ho, M.-R., Wang, I., Hsu, S.-T. D., Guo, R.-T., Chang, W., Wang, A. H.-J.* Crystal structure of vaccinia viral A27 protein reveals a novel structure critical for its function and complex formation with A26 protein. PLoS Pathogens, 2013, 9(8): e1003563.
122. Ren, F., #Ko, T.-P., #Feng, X., Huang, C.-H., Chan, H.-C., Hu, Y., Wang, K., Ma, Y., Liang, P.-H., Wang, A. H.-J., Oldfield, E.,* Guo, R.-T. * Insights into the mechanism of the antibiotic-synthesizing enzyme MoeO5 from crystal structures of different complexes. Angewandte Chemie International Edition, 2012, 51(17): 4157-4160. (Cover story)
123. Zheng, Y., Huang, C.-H., Liu, W., Ko, T.-P., Xue, Y., Zhou, C., Guo, R.-T., Ma, Y.* Crystal structure and substrate-binding mode of a novel pectate lyase from alkaliphilic Bacillus sp. N16-5. Biochemical and B iophysical R esearch C ommunications , 2012, 420(2): 269-274.
124. Liu, W., Sun, Y., Ko, T.-P., Wiegel, J., Shao, W., Lu, F., Guo, R.-T., Huang, C.-H.* Crystallization and preliminary X-ray diffraction analysis of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485. Acta C rystallographica. Section F, Structural B iology and C rystallization C ommunications , 2012, 68: 914-916.
125. Huang, J.-W., Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Lai, H.-L., Chen, C.-C., Ma, Y., Zheng, Y., Huang, C.-H., Zou, P., Liu, J.-R.,* Guo, R.-T.* Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase. Applied M icrobiology and B iotechnology , 2012, 94(1): 111-121.
126. Huang, C.-H., # Sun, Y., # Ko, T.-P., Chen, C.-C., Zheng, Y., Chan, H.-C., Pang, X., Wiegel, J.,* Shao, W.,* Guo, R.-T.* The substrate/product-binding modes of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485. The Biochemical J ournal , 2012, 448(3): 401-407.
127. Cheng, Y.-S., Ko, T.-P., Huang, J.-W., Wu, T.-H., Lin, C.-Y., Luo, W., Li, Q., Ma, Y., Huang, C.-H., Wang, A. H.-J., Liu, J.-R.,* Guo, R.-T.* Enhanced activity of Thermotoga maritima cellulase 12A by mutating a unique surface loop. Applied M icrobiology and B iotechnology , 2012, 95(3): 661-669.
128. Chang, K.-M., Chen, S.-H., Kuo, C.-J., Chang, C.-K., Guo, R.-T., Yang, J. M., Liang, P.-H.* Roles of amino acids in the Escherichia coli octaprenyl diphosphate synthase active site probed by structure-guided site-directed mutagenesis. Biochemistry, 2012, 51(16): 3412-3419.
129. Chan, H.-C., # Zhu, Y., # Hu, Y., Ko, T.-P., Huang, C.-H., Ren, F., Chen, C.-C., Ma, Y., Guo, R.-T.,* Sun, Y.* Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars. Protein & C ell , 2012, 3(2): 123-131. (Cover story)
130. Pai, C. K. Wang, H. T., Guo, R.-T. , Liu, J.-R.* The construction of bifunctional fusion xylanolytic enzymes and the prediction of optimum reaction conditions for the enzyme activity. BioResources, 2012, 7(4): 5647-5665.
131. Zeng, Y.-F., # Ko, T.-P., # Lai, H.-L., Cheng, Y.-S., Wu, T.-H., Ma, Y., Chen, C.-C., Yang, C. S., Cheng, K. J., Huang, C.-H., Guo, R.-T.,* Liu, J.-R.* Crystal structures of Bacillus alkaline phytase in complex with divalent metal ions and inositol hexasulfate. Journal of M olecular B iology , 2011, 409(2): 214-224. (Cover story)
132. Wu, T.-H., # Huang, C.-H., # Ko, T.-P., Lai, H.-L., Ma, Y., Chen, C.-C., Cheng, Y.-S., Liu, J.-R.,* Guo, R.-T.* Diverse substrate recognition mechanism revealed by Thermotoga maritima Cel5A structures in complex with cellotetraose, cellobiose and mannotriose. Biochimica et B iophysica A cta , 2011, 1814(12): 1832-1840. (Cover story)
133. Tseng, C.-W., # Ko, T.-P., # Guo, R.-T., # Huang, J.-W., Wang, H.-C., Huang, C.-H., Cheng, Y.-S., Wang, A. H.-J., Liu, J.-R.* Substrate binding of a GH5 endoglucanase from the ruminal fungus Piromyces rhizinflata. Acta C rystallographica. Section F, Structural B iology and C rystallization C ommunications , 2011, 67: 1189-1194. (Cover story)
134. Cheng, Y.-S., Ko, T.-P., Wu, T.-H., Ma, Y., Huang, C.-H., Lai, H.-L., Wang, A. H.-J., Liu, J.-R.,* Guo, R.-T.* Crystal structure and substrate-binding mode of cellulase 12A from Thermotoga maritima. Proteins, 2011, 79(4): 1193-1204. (Cover story)
135. Zhang, Y., Cao, R., Yin, F., Hudock, M. P., Guo, R.-T., Krysiak, K., Mukherjee, S., Gao, Y.-G., Robinson, H., Song, Y., No, J.-H., Bergan, K., Leon, A., Cass, L., Goddard, A., Chang, T.-K., Lin, F.-Y., Van Beek, E., Papapoulos, S., Wang, A. H.-J., Kubo, T., Ochi, M., Mukkamala, D., Oldfield, E.* Lipophilic bisphosphonates as dual farnesyl/geranylgeranyl diphosphate synthase inhibitors: an X-ray and NMR investigation. Journal of the American Chemical Society, 2009, 131(14): 5153-5162.
136. Guo, R.-T., # Chong, Y.-E., # Guo, M., Yang, X.-L.* Crystal structures and biochemical analyses suggest a unique mechanism and role for human glycyl-tRNA synthetase in Ap4A homeostasis. The Journal of B iological C hemistry , 2009, 284(42): 28968-28976.
137. Chen, C.-K., Lee, G.-C., Ko, T.-P., Guo, R.-T., Huang, L.-M., Liu, H.-J., Ho, Y.-F., Shaw, J.-F., Wang, A. H.-J.* Structure of the alkalohyper thermophilic Archaeoglobus fulgidus lipase contains a unique C-terminal domain essential for long-chain substrate binding. Journal of M olecular B iology , 2009, 390(4): 672-685.
138. Wu, Y.-H., Ko, T.-P., Guo, R.-T., Hu, S.-M., Chuang, L.-M., Wang, A. H.-J.* Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2. Structure, 2008, 16(11): 1714-1723.
139. Kuo, C.-J., Guo, R.-T., Lu, I.-L., Liu, H.-G., Wu, S.-Y., Ko, T.-P., Wang, A. H.-J., Liang, P.-H.* Structure-based inhibitors exhibit differential activities against Helicobacter pylori and Escherichia coli undecaprenyl pyrophosphate synthases. Journal of B iomedicine & B iotechnology , 2008, 2008:841312.
140. Jeng, W.-Y., Ko, T.-P., Liu, C.-I., Guo, R.-T., Liu, C.-L., Shr, H.-L., Wang, A. H.-J.* Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation in Staphylococcus epidermidis. Nucleic A cids R esearch , 2008, 36(5): 1567-1577.
141. Cao, R., Chen, C.-K. C., Guo, R.-T., Wang, A. H.-J.,* Oldfield, E.* Structures of a potent phenylalkyl bisphosphonate inhibitor bound to farnesyl and geranylgeranyl diphosphate synthases. Proteins, 2008, 73(2): 431-439.
142. Chen, K.-M. C., Hudock, M. P., Zhang, Y., Guo, R.-T., Cao, R., No, J.-H., Liang, P.-H., Ko, T.-P., Chang, T.-H., Chang, S.-C., Song, Y., Axelson, J., Kumar, A., Wang, A. H.-J., Oldfield, E.* Inhibition of geranylgeranyl diphosphate synthase by bisphosphonates: a crystallographic and computational investigation. Journal of M edicinal C hemistry , 2008, 51(18): 5594-5607.
143. Guo, R.-T., # Cao, R., # Liang, P.-H., Ko, T.-P., Chang, T.-H., Hudock, M. P., Jeng, W.-Y., Chen, C.-K., Zhang, Y., Song, Y., Kuo, C.-J., Yin, F., Oldfield, E., Wang, A. H.-J.* Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases. Proceedings of the N ational A cademy of S ciences of the U nited S tates of A merica , 2007, 104(24): 10022-10027.
144. Chang, T.-H., Guo, R.-T., Ko, T.-P., Wang, A. H.-J.,* Liang, P.-H.* Crystal structure of type-III geranylgeranyl pyrophosphate synthase from Saccharomyces cerevisiae and the mechanism of product chain length determination. The Journal of B iological C hemistry , 2006, 281(21): 14991-15000.
145. Sun, H.-Y., Ko, T.-P., Kuo, C.-J., Guo, R.-T., Chou, C.-C., Liang, P.-H.,* Wang, A. H.-J.* Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures. Journal of B acteriology , 2005, 187(23): 8137-8148.
146. Guo, R.-T., # Ko, T.-P., # Chen, A. P., Kuo, C.-J., Wang, A. H.-J.,* Liang, P.-H.* Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis. The Journal of B iological C hemistry , 2005, 280(21): 20762-20774.
147. Guo, R.-T., # Kuo, C.-J., # Ko, T.-P., Chou, C.-C., Liang, P.-H.,* Wang, A. H.-J.* A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long chain trans-prenyl products. Biochemistry, 2004, 43(24): 7678-7686.
148. Guo, R.-T., Kuo, C.-J., Chou, C.-C., Ko, T.-P., Shr, H.-L., Liang, P.-H.,* Wang, A. H.-J.* Crystal structure of octaprenyl pyrophosphate synthase from hyperthermophilic Thermotoga maritima and mechanism of product chain length determination. The Journal of B iological C hemistry , 2004, 279(6): 4903-4912.
149. Chu, H.-M., # Guo, R.-T., # Lin, T. W., Chou, C.-C., Shr, H.-L., Lai, H.-L., Tang, T.-Y., Cheng, K.-J., Selinger, B. L., Wang, A. H.-J.* Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis. Structure, 2004, 12(11): 2015-2024.
150. Guo, R.-T., # Ko, T.-P., # Chou, C.-C., Shr, H.-L., Chu, H.-M., Tsai, Y.-H., Liang, P.-H., Wang, A. H.-J.* Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals from Thermotoga maritima and Escherichia coli. Acta C rystallographica. Section D, Biological C rystallography , 2003, 59: 2265-2268.
151. Guo, R.-T., Chou, L.-J., Chen, Y.-C., Chen, C.-Y., Pari, K., Jen, C. J., Lo, S. J., Huang, S.-L., Lee, C.-Y., Chang, T.-W., Chuang, W.-J.*. Expression in Pichia pastoris and characterization by circular dichroism and NMR of rhodostomin. Proteins, 2001, 43(4): 499-508.
六、 已授权专利
1. 陈纯琪,黄建文,戴隆海,喻雪婧,陈青谕,吴姗,张丽兰,翟超,马立新, 郭瑞庭。酶活性提升的纤维素酶,授权号:CN111826367B,中国专利。
2. 郭瑞庭,韩旭,郑迎迎,刘卫东,商娜,黄建文,李鲜,戴隆海,杨钰,刘珂,陈纯琪。具提升活性的PET水解酶,授权号:CN114480343B,中国专利。
3. 陈纯琪,黄建文,栗秀琴,曾伟,张丽兰,杨云云,戴隆海,闵鉴,杨钰, 郭瑞庭。具有提升的热稳定性的PET降解酶,授权号:CN115125225B,中国专利。
4. 郭瑞庭,郑雅珊,吴姿慧,黄建文,赖惠琳,林正言。一种纤维素酶及其应用,授权号:ZL2011100426000,中国专利。
5. 郭瑞庭,黄建文,郑雅珊,吴姿慧,赖惠琳,林正言。酶活性及热稳定性被提高的葡聚糖酶,授权号:ZL2011100596815,中国专利。
6. 郭瑞庭,黄建文,郑雅珊,吴姿慧,赖惠琳,林正言,黄婷沅。提升酶活性之木聚糖酶,授权号:ZL2013101344011,中国专利。
7. 郭瑞庭,黄建文,郑雅珊,吴姿慧,赖惠琳,林正言,黄婷沅。酶活性提高的甘露聚糖酶,授权号:ZL201310135208X,中国专利。
8. 郭瑞庭,陈纯琪,吴姿慧,郑雅珊,黄建文,赖惠琳,林正言,黄婷沅。具有提升的酶活性的植酸酶,授权号:CN104342418B,中国专利。
9. 郭瑞庭,陈纯琪,吴姿慧,郑雅珊,黄建文,赖惠琳,林正言,黄婷沅。具有提升的酶活性的植酸酶,授权号:CN106399273B,中国专利。
10. Chen, C.-C., Huang, J.-W., Dai, L., Yu, X., Chen, C.-Y., Wu S., Zhan, Z., Zhang, L., Zhai, C., Ma, L., Guo, R.-T. Cellulase having improved enzymatic activity,,授权号:US10479985B1,美国专利。
11. Chen, C.-C., Huang, J.-W., Li, X., Zeng, W., Zhang, L., Yang, Y., Dai, L., Min, J., Yang, Y., Guo, R.-T. PET hydrolase having improved thermal stability, 授权号:US11680252,美国专利。
12. Guo, R.-T., Zheng, Y., Xu, Z. , Chen, C.-C., Peng, W., Liu, W., Liu, J.-R., Ma, Y. Lactone hydrolase and method of degrading alpha-zearalenol using the same,授权号:US10316303B2,美国专利。
13. Guo, R.-T.,Cheng, Y.-S., Wu, T.-H., Huang, J.-W., Lai, H.-L., Lin, C.-Y.,Thermostable cellulase having increased enzyme activity,授权号:US8137945B1,美国专利。
14. Guo, R.-T., Huang, J.-W., Cheng, Y.-S., Wu, T.-H., Lai, H.-L., Lin C.-Y.,Glucanase having increased enzyme activity and thermostability,授权号:US8192974B1,美国专利。
15. Guo, R.-T., Cheng, Y.-S., Huang, J.-W., Wu, T.-H., Lai, H.-L., Lin, C.-Y., Huang, T.-Y.,Xylanase having improved enzymatic activity,授权号:US8722382B1,美国专利。
16. Guo, R.-T.,Huang, J.-W., Cheng, Y.-S., Wu, T.-H., Lai, H.-L., Lin, C.-Y., Huang, T.-Y.,Beta-mannanase having improved enzymatic activity,授权号:US8865444B1,美国专利。
17. Guo, R.-T.,Huang, J.-W., Cheng, Y.-S., Wu, T.-H., Lai, H.-L., Lin C.-Y., Huang T.-Y. β-mannanase having improved production yield and enzymatic activity,授权号:US8999695B1,美国专利。
18. Guo, R.-T.,Wu, T.-H., Cheng, Y.-S., Huang, J.-W., Lai, H.-L., Lin C.-Y., Huang T.-Y. Phytase having improved enzymatic activity,授权号:US9194010B2,美国专利。
19. Chen, C.-C., Huang, J.-W., Dai, L., Yu, X., Chen, C.-Y., Wu, S., Zhan, Z., Zhang, L., Zhai, C., Ma, L., Guo, R.-T. Cellulase having improved enzymatic activity,授权号:US10479985B1,美国专利。
20. Guo, R.-T.,Chen, C.-C., Cheng, Y.-S., Huang, J.-W., Wu, T.-H., Lai, H.-L., Lin, C.-Y., Ko, T.-Y. Cellulase having improved enzymatic activity,授权号:US9809807B1,美国专利。
21. Guo R.-T., Cheng Y.-S., Huang J.-W., Wu T.-H., Lai H.-L., Lin C.-Y., Ko T.-Y. Cellulase having improved thermostability, 授权号:US9944915B2,美国专利。
22. Wu, T.-H., Cheng, Y.-S., Lai, H.-L., Ke, T.-Y., Huang, J.-W., Chen, C.-C., Guo, R.-T. Phytase having improved thermostability,授权号:US10011822B2,美国专利。
23. Cheng, Y.-S., Wu, T.-H., Lin, C.-Y., Lai, H.-L., Zheng, C.-B., Huang, T.-Y., Lin, I.-H., Huang, J.-W., Chen, C.-C., Guo, R.-T. Glucose oxidase having improved thermostability,授权号:US10233430B1,美国专利。
24. Chen, C.-C., Wang, T., Huang, J.-W., Guo, R.-T. Lysozyme having improved enzymatic activity,授权号:US10323237B2,美国专利。
25. Cheng, Y.-S., Wu, T.-H., Lin, C.-Y., Lai, H.-L., Zheng, C.-B., Huang, T.-Y., Lin, I.-H., Huang, J.-W., Chen, C.-C., Guo, R.-T. Xylosidase having improved enzymatic activity,授权号:US10385346B2,美国专利。
七、获奖与荣誉称号
1. 2020年,“湖北省创新群体”(群体负责人)
2. 2019年,“湖北省省级人才入选者”
3. 2019年,“天津市自然科学二等奖”(第一完成人)
4. 2018年,“国家级人才入选者”
5. 2017年,“部级人才入选者”
6. 2015年,“天津市131第一层次人才培养工程”
7. 2012年,“天津市131第二层次人才培养工程”
8. 2012年,“2011年科学中国人年度人物”
9. 2010年,“中国科学院人才计划入选者”,择优支持
10. 2010年,“天津市市级人才计划入选者”,为天津市特聘教授
2021 年以湖北大学图书馆为背景发表的 P450 综述文章
获选的其中 25 篇封面文章
