一、基本信息

李壮，博士

电子邮件：zhuangli@hubu.edu.cn

研究领域：可编程核酸酶的发现、生化性质、及工作机理

实验室网站：zhuangli200.github.io

二、教育背景

2012.09–2018.01 北京大学，博士

2008.09–2012.07 西北大学，本科

三、工作经历

2021.10至今 湖北大学，生命科学学院

2021.03–2021.09 哥伦比亚大学，博士后科学家

2018.03–2021.03 普渡大学，博士后

四、论文(*第一作者，#通讯作者)

Programmable Nucleases:

1. Wang, L.*, Chen, W.*, Zhang, C.*, Xie, X., Huang, F., Chen, M., Mao,W., Yu, N., Wei, Q., Ma, L.#, Li, Z.#. Molecular mechanism for target recognition, dimerization, and activation of Pyrococcus furiosus Argonaute. Molecular Cell. 2024.

2. Wang, X.*, Li, X.*, Yu, G.*, Zhang, L.*, Zhang, C.*, Wang, L.*, Liao, F., Wen, Y., Yin, H., Liu, X., Wei, Y., Li, Z.#, Deng, Z.#, Zhang, H.#. Structural insights into mechanisms of Argonaute protein-associated NADase activation in bacterial immunity. Cell Research. 2023.

3. Li, X.*, Liao, F.*, Gao, J., Song, G., Zhang, C., Ji, N., Wang, X., Wen, J., He, J., Wei, Y., Zhang, H., Li, Z.#, Yu, G.#, Yin, H.#. Inhibitory mechanism of CRISPR-Cas9 by AcrIIC4. Nucleic Acids Research. 2023.

4. Wang, F.*, Zhang, C.*, Xu, H., Zeng, W., Ma, L., Li, Z.#. Structural Basis for the Ribonuclease Activity of a Thermostable CRISPR-Cas13a from Thermoclostridium caenicola. Journal of Molecular Biology. 2023.

5. Xiao, R.*, Wang, S.*, Han, R., Li, Z., Gabel, C., Mukherjee I., and Chang, L.#. Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition. Molecular Cell. 2021.

6. Li, Z.*, Zhang, H.*, Xiao, R.*, Han, R., and Chang, L.#. Cryo-EM structure of the RNA-guided ribonuclease Cas12g. Nature Chemical Biology. 2021.

7. Xiao, R.*, Li, Z.*, Wang, S., Han, R. and Chang, L.#. Structural basis for substrate recognition and cleavage by the dimerization-dependent CRISPR-Cas12f nuclease. Nucleic Acids Research. 2021.

8. Gabel, C.*, Li, Z.*, Zhang, H., and Chang, L.#. Structural basis for inhibition of the type I-F CRISPR-Cas surveillance complex by AcrIF4, AcrIF7, and AcrIF14. Nucleic Acids Research. 2020.

9. Zhang, H.*, Li, Z.*, Xiao, R.*, and Chang, L.#. Mechanisms for target recognition and cleavage by the Cas12i RNA-guided endonuclease. Nat. Struct. Mol. Biol. 2020.

10. Li, Z.*, Zhang, H.*, Xiao, R., and Chang, L.#. Cryo-EM structure of a type I-F CRISPR RNA guided surveillance complex bound to transposition protein TniQ. Cell Research. 2020.

11. Zhang, H.*, Li, Z.*, Daczkowski, C.M., Gabel, C., Mesecar, A.D., and Chang, L.#. Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins. Cell Host & Microbe. 2019.

Others:

12. Ma, Y.*, Li, X.*, Wang, F.*, Zhang, L., Zhou, S., Che, X., Yu, D., Liu, X., Li, Z.#, Sun, H.#, Yu, G.#, Zhang, H.#. Structural and biochemical characterization of the key components of an auxin degradation operon from the rhizosphere bacterium Variovorax. Plos Biology. 2023.

13. Huang, J.*, Liu, X.*, Sun, Y.*, Li, Z., Lin, M., Hamilton, K., Mandel, C., Sandmeir, F., Conti, E., Oyala, P., Tong, L.#. An examination of the metal ion content in the active sites of human endonucleases CPSF73 and INTS11. Journal of Biological Chemistry. 2023.

14. Liu, J., Li, B., Li, Z., Yang, F., Chen, B., Chen, J., Li, H., Jiang, Z.#. Deciphering the alkaline stable mechanism of bacterial laccase from Bacillus pumilus by molecular dynamics simulation can improve the decolorization of textile dyes. Journal of Hazardous Materials. 2023.

15. Yin, H.*, Zheng, J.*, He, Q.*, Zhang, X., Li, X., Ma, Y., Liang, X., Gao, J., Kocsis, B., Li, Z., Liu, X., Alto, N., Li, L.#, Zhang, H.#. Insights into the GSDMB-mediated cellular lysis and its targeting by IpaH7.8. Nature Communications. 2023.

16. Gabel, C., Li, Z., DeMarco, A., Zhang, Z., Yang, J., Hall, M., Barford, D., Chang, L.#. Molecular architecture of the augmin complex. Nature Communications. 2022.

17. Chen, Q., Plasencia, M., Li, Z., Mukherjee, S., Patra, D., Chen, C., Klose, T., Yao, X., Kossiakoff, A., Chang, L., Andrews, P., Tesmer, J.#. Structure of a G protein-coupled receptor kinase engaging its GPCR. Nature. 2021.

18. Guntupalli, S. R.*, Li, Z.*, Chang, L., Plapp, B. V., Subramanian, R.#. Cryo-Electron Microscopy Structures of Yeast Alcohol Dehydrogenase. Biochemistry. 2021.

19. Li, Z., Zhao, D., Xiang, B., and Li, H.#. Structural and biochemical characterization of DAXX-ATRX interaction. Protein & Cell. 2017.